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Title: Molecular Biology and pathogenesis of coronavirus: Virus and host factors involved in viral RNA transcription
Keywords: coronavirus,RNA binding,IBV,RNA synthesis,nsp8,nsp12
Issue Date: 18-Jan-2012
Citation: TAN YONG WAH (2012-01-18). Molecular Biology and pathogenesis of coronavirus: Virus and host factors involved in viral RNA transcription. ScholarBank@NUS Repository.
Abstract: Coronaviruses RNA synthesis is regulated by both virus-encoded and cellular proteins. In a screen based on a yeast three-hybrid system using the 5?-untranslated region (5?-UTR) of SARS coronavirus (SARS-CoV) RNA as bait against the HeLa cell cDNA library, zinc finger CCHC-type and RNA binding motif 1 (MADP1) was isolated and subsequently confirmed in coronavirus infectious bronchitis virus (IBV). The specificity of the interaction was determined and the interacting regions were subsequently mapped to the N-terminal portion of MADP1 and stem loop I of IBV 5?-UTR. MADP1 was also found to be translocated to the cytoplasm in IBV-infected cells using indirect immunofluorescence and defective viral RNA synthesis had been observed in MADP1 knockdown cells. The IBV non-structural protein 8 (nsp8) was found to interact with nsp12 at the latter?s N- and C-terminal regions and a possible mode of cooperation between the two replicase gene products had been proposed.
Appears in Collections:Ph.D Theses (Open)

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