Please use this identifier to cite or link to this item:
Title: Molecular Biology and pathogenesis of coronavirus: Virus and host factors involved in viral RNA transcription
Keywords: coronavirus,RNA binding,IBV,RNA synthesis,nsp8,nsp12
Issue Date: 18-Jan-2012
Source: TAN YONG WAH (2012-01-18). Molecular Biology and pathogenesis of coronavirus: Virus and host factors involved in viral RNA transcription. ScholarBank@NUS Repository.
Abstract: Coronaviruses RNA synthesis is regulated by both virus-encoded and cellular proteins. In a screen based on a yeast three-hybrid system using the 5?-untranslated region (5?-UTR) of SARS coronavirus (SARS-CoV) RNA as bait against the HeLa cell cDNA library, zinc finger CCHC-type and RNA binding motif 1 (MADP1) was isolated and subsequently confirmed in coronavirus infectious bronchitis virus (IBV). The specificity of the interaction was determined and the interacting regions were subsequently mapped to the N-terminal portion of MADP1 and stem loop I of IBV 5?-UTR. MADP1 was also found to be translocated to the cytoplasm in IBV-infected cells using indirect immunofluorescence and defective viral RNA synthesis had been observed in MADP1 knockdown cells. The IBV non-structural protein 8 (nsp8) was found to interact with nsp12 at the latter?s N- and C-terminal regions and a possible mode of cooperation between the two replicase gene products had been proposed.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
TanYW.pdf30.2 MBAdobe PDF



Page view(s)

checked on Dec 11, 2017


checked on Dec 11, 2017

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.