Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34353
Title: TRANSCRIPTION FACTOR ZIC3 CONTROLS CELL FATE DECISIONS
Authors: FELICIA HONG HUIMEI
Keywords: Zic3, pluripotency, differentiation, embryonic stem cell, lineage specification, combinatorial binding of transcription factors
Issue Date: 18-Jan-2012
Source: FELICIA HONG HUIMEI (2012-01-18). TRANSCRIPTION FACTOR ZIC3 CONTROLS CELL FATE DECISIONS. ScholarBank@NUS Repository.
Abstract: Zic3 plays essential roles in development and the implicated cell types are primarily derived from the mesoderm and neuroectoderm. It was previously reported that Zic3 is required for pluripotency by repressing the endodermal lineage. I show that Zic3 directly activates genes involved in negative regulation of differentiation, early mesodermal and neuroectodermal development, while directly repressing genes involved in late differentiation. Zic3 co-occupies genes with other ES cell regulators in a lineage-specific fashion. Further investigation of Zic3 in pluripotency and cell fate specification showed that Zic3 enhances the ES cell program, and directly activates Nanog, one of the core ES cell transcription factors. Similar to Nanog, Zic3 is required for germ cell migration. However, unlike Nanog but more similar to Oct4 and Sox2, the overexpression of Zic3 promotes cell fate specification. Overexpression of Zic3 precludes endodermal specification, and enhances mesodermal and neuroectodermal specification thereby indicating that Zic3 controls cell fate decision.
URI: http://scholarbank.nus.edu.sg/handle/10635/34353
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