Please use this identifier to cite or link to this item: https://doi.org/10.1016/0041-0101(94)90083-3
Title: Pharmacological effects and pathological changes induced by the venom of Pseudechis australis in isolated skeletal muscle preparations
Authors: Chen, S.Z.J.
Gwee, M.C.E. 
Gopalakrishnakone, P. 
Issue Date: 1994
Citation: Chen, S.Z.J., Gwee, M.C.E., Gopalakrishnakone, P. (1994). Pharmacological effects and pathological changes induced by the venom of Pseudechis australis in isolated skeletal muscle preparations. Toxicon 32 (3) : 303-315. ScholarBank@NUS Repository. https://doi.org/10.1016/0041-0101(94)90083-3
Abstract: The venom from the Australian king brown snake Pseudechis australis produced a concentration-dependent and progressive inhibition of directly and indirectly evoked twitches of the mouse hemidiaphragm: 2, 5 and lO μg/ml of venom produced complete blockade of directly evoked twitches after 87 ± 26 min, 66 ± 28 min and 56 ± 13 min, respectively, whereas twitches elicited by indirect stimulation were blocked after 43 ± 4 min, 34 ± 2 min and 21 ± 2 min, respectively. Two, 5 and 10 μg/ml of the venom also progressively inhibited the twitch responses of the chick biventer cervicis muscle evoked by nerve stimulation: the times (min) to 90% blockade of the twitches were 127 ± 12, 41 ± 8 and 26 ± 6, respectively. Light and electron microscopic studies confirmed that the venom caused severe muscle damage and necrosis in the chick and mouse skeletal muscle. The degree of muscle damage was proportional to the concentration of venom used. The muscle degeneration was characterized by disorganized myofibrils, dilatation of the sarcoplasmic reticulum and swollen or brokendown mitochondria. Depletion and a decrease in population of storage vesicles in the neuromuscular junction were also observed. Thus, the inhibition of neuromuscular function by the venom of P. australis was a consequence of the extensive morphological damage to muscle fibres, motor nerve terminals and cytoplasmic organelles which could be attributed to a direct myotoxic action presumably mediated by the enzymatic action of phospholipase A2 present in the venom of P. australis.
Source Title: Toxicon
URI: http://scholarbank.nus.edu.sg/handle/10635/33918
ISSN: 00410101
DOI: 10.1016/0041-0101(94)90083-3
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

15
checked on Nov 11, 2018

WEB OF SCIENCETM
Citations

14
checked on Oct 23, 2018

Page view(s)

120
checked on Oct 13, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.