Cloning and characterization of cDNAs encoding three isoforms of phospholipase A2in Malayan spitting cobra (Naja naja sputatrix) venom

Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0041-0101(96)00071-2

Title:	Cloning and characterization of cDNAs encoding three isoforms of phospholipase A2in Malayan spitting cobra (Naja naja sputatrix) venom
Authors:	Armugam, A. Tan, N.H. Earnest, L. Chung, M.C.M. Tan, C.H. Jeyaseelan, K. Gopalakrishnakone, P.
Issue Date:	1997
Citation:	Armugam, A., Tan, N.H., Earnest, L., Chung, M.C.M., Tan, C.H., Jeyaseelan, K., Gopalakrishnakone, P. (1997). Cloning and characterization of cDNAs encoding three isoforms of phospholipase A2in Malayan spitting cobra (Naja naja sputatrix) venom. Toxicon 35 (1) : 27-37. ScholarBank@NUS Repository. https://doi.org/10.1016/S0041-0101(96)00071-2
Abstract:	cDNAs encoding three phospholipase A2 (PLA2) isoforms in Naja naja sputatrix were cloned and characterized. One of them encoded an acidic PLA2 (APLA) while the others encoded neutral PLA2 (NPLA-1 and NPLA-2). The specific characteristics of APLA and NPLA were attributed to mutations at nt139 and nt328 from G to C and G to A, respectively, resulting in amino acid substitutions from Asp20 and 83 in APLA to His20 and Asn83 in NPLA. Amino acid sequencing of purified protein also showed the presence of this Asp20 and His20 in APLA and NPLA, respectively. The cDNA encoding one of the PLA2 (NAJPLA-2A), when expressed in Escherichia coli, yielded a protein that exhibited PLA2 activity.
Source Title:	Toxicon
URI:	http://scholarbank.nus.edu.sg/handle/10635/33909
ISSN:	00410101
DOI:	10.1016/S0041-0101(96)00071-2
Appears in Collections:	Staff Publications

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