Please use this identifier to cite or link to this item:
|Title:||Acidification-and-recovery induces nuclear accumulation of neutral red and DNA into human KB oral carcinoma cells|
|Authors:||Sit, K.H. |
pGemβgal plasmid lacZ
|Source:||Sit, K.H.,Bay, B.H.,Paramanantham, R.,Tana, H.M.,Wong, K.P. (1996). Acidification-and-recovery induces nuclear accumulation of neutral red and DNA into human KB oral carcinoma cells. Cancer Letters 104 (1) : 63-69. ScholarBank@NUS Repository. https://doi.org/10.1016/0304-3835(96)04223-1|
|Abstract:||In cultures of oral cancers, gene transfer has been achieved by delivery systems which introduce DNA into cells but not specifically into the nucleus. We observed that acidification and recovery converted the nuclear-insulated interphase KB carcinoma cells into intense nuclear-accumulating states. Nuclear sequestration of the vital dye neutral red and T7 oligonucleotide was demonstrated qualitatively, and quantitatively by image analysis of single cells. pGemβgal plasmids of 6.8 kb were introduced into KB cells by similar acidification and recovery pulses. Successful integration into the host KB genome was shown by expression of the lacZ gene of the plasmids. Enhanced nucleo-cytoplasmic transport demonstrated here in KB oral epidermoid carcinoma cells could potentially facilitate gene therapy in oral cancers.|
|Source Title:||Cancer Letters|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 5, 2017
WEB OF SCIENCETM
checked on Nov 2, 2017
checked on Dec 9, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.