Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0006-2952(01)00854-1
Title: Biochemical and pharmacological characterization of the venom of the black scorpion Heterometrus spinifer
Authors: Nirthanan, S. 
Cheah, L.-S.
Gwee, M.C.E. 
Joseph, J.S.
Khoo, H.-E. 
Gopalakrishnakone, P. 
Keywords: Acetylcholine
Electrospray ionization-mass spectrometry/mass spectrometry
Heterometrus spinifer
Norepinephrine
Scorpion venom
Issue Date: 2002
Source: Nirthanan, S., Cheah, L.-S., Gwee, M.C.E., Joseph, J.S., Khoo, H.-E., Gopalakrishnakone, P. (2002). Biochemical and pharmacological characterization of the venom of the black scorpion Heterometrus spinifer. Biochemical Pharmacology 63 (1) : 49-55. ScholarBank@NUS Repository. https://doi.org/10.1016/S0006-2952(01)00854-1
Abstract: The sting of the black scorpion Heterometrus spinifer, which can cause intense localized pain, has not been reported to produce lethal cardiovascular complications, which are well known to result from scorpion envenomation as a consequence of a massive release of catecholamines. Therefore, we have undertaken a biochemical and pharmacological characterization of the venom of H. spinifer. Pharmacologically, the venom (0.125 μL/mL) produced a marked, reversible contracture in the chick biventer cervicis muscle that was blocked by d-tubocurarine (2 μM) but not by tetrodotoxin (5 μM) and ω-conotoxin GVIA (3 μM). The anticholinesterase neostigmine (1 μM) potentiated the contracture by 5.3-fold. An ultra-filtrate fraction of MW < 3000 (F3K) of the venom produced a similar contracture in the biventer muscle, whereas the retentate of MW > 3000 did not. In the rat anococcygeus muscle, the venom produced a contractile response that was partially (37.4 ± 1.6%) blocked by atropine (5 μM); phentolamine (5 μM) blocked the remaining response. Tetrodotoxin (5 μM) did not block the contractile response of the venom on the anococcygeus muscle. Electrospray ionization-mass spectrometry/mass spectrometry confirmed the presence of high concentrations of acetylcholine (79.8 ± 1.7 μM) and norepinephrine (146.7 ± 19.8 μM) in H. spinifer venom, which can fully account for the observed cholinergic and adrenergic effects. In contrast to scorpion venoms that selectively target neuronal ion channels in mediating transmitter release, our data show that H. spinifer venom does not possess such activity, which likely explains the apparent lack of lethality of black scorpion envenomation.
Source Title: Biochemical Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/33898
ISSN: 00062952
DOI: 10.1016/S0006-2952(01)00854-1
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

16
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

11
checked on Nov 29, 2017

Page view(s)

165
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.