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|Title:||Purification and partial characterization of stonustoxin (lethal factor) from Synanceja horrida venom|
|Citation:||Poh, C.H., Yuen, R., Khoo, H.E., Chung, M., Gwee, M., Gopalakrishnakone, P. (1991). Purification and partial characterization of stonustoxin (lethal factor) from Synanceja horrida venom. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 99 (4) : 793-798. ScholarBank@NUS Repository. https://doi.org/10.1016/0305-0491(91)90143-2|
|Abstract:||1. The lethal factor of the stonefish (Synanceja horrida) venom, designated as the stonustoxin, was purified to homogeneity by a two-step procedure on Sephacryl S-200 High Resolution (HR) gel permeation and DEAE Bio-Gel A anion exchange chromatography. 2. Stonustoxin has a native mol. wt of 148,000 and an isoelectric point of 6.9. 3. SDS-polyacrylamide gel electrophoresis revealed two subunits (designated α and β) with mol. wts of 71,000 and 79,000, respectively. 4. The amino acid composition of both subunits and the N-terminal amino acid sequence of the β subunit were also determined. 5. Purified stonustoxin had an LD50 of 0.017 μg/g which is 22-fold more potent than that of the crude venom. 6. The toxin exhibited potent haemolytic activity in vitro and endema-inducing activity with a minimum edema dose (MED) of 0.15 μg in mouse paw. The edema effect was not antagonized by diphenhydramine.|
|Source Title:||Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology|
|Appears in Collections:||Staff Publications|
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