Please use this identifier to cite or link to this item: https://doi.org/10.1016/0305-0491(91)90143-2
Title: Purification and partial characterization of stonustoxin (lethal factor) from Synanceja horrida venom
Authors: Poh, C.H.
Yuen, R. 
Khoo, H.E. 
Chung, M. 
Gwee, M. 
Gopalakrishnakone, P. 
Issue Date: 1991
Source: Poh, C.H., Yuen, R., Khoo, H.E., Chung, M., Gwee, M., Gopalakrishnakone, P. (1991). Purification and partial characterization of stonustoxin (lethal factor) from Synanceja horrida venom. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 99 (4) : 793-798. ScholarBank@NUS Repository. https://doi.org/10.1016/0305-0491(91)90143-2
Abstract: 1. The lethal factor of the stonefish (Synanceja horrida) venom, designated as the stonustoxin, was purified to homogeneity by a two-step procedure on Sephacryl S-200 High Resolution (HR) gel permeation and DEAE Bio-Gel A anion exchange chromatography. 2. Stonustoxin has a native mol. wt of 148,000 and an isoelectric point of 6.9. 3. SDS-polyacrylamide gel electrophoresis revealed two subunits (designated α and β) with mol. wts of 71,000 and 79,000, respectively. 4. The amino acid composition of both subunits and the N-terminal amino acid sequence of the β subunit were also determined. 5. Purified stonustoxin had an LD50 of 0.017 μg/g which is 22-fold more potent than that of the crude venom. 6. The toxin exhibited potent haemolytic activity in vitro and endema-inducing activity with a minimum edema dose (MED) of 0.15 μg in mouse paw. The edema effect was not antagonized by diphenhydramine.
Source Title: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/33875
ISSN: 03050491
DOI: 10.1016/0305-0491(91)90143-2
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