Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2003.11.020
Title: Optical immunoassay for snake venom detection
Authors: Van, Dong L.
Quyen, L.K.
Gopalakrishnakone, P. 
Eng, K.H. 
Keywords: Bovine serum albumin
BSA
ELISA
Enzyme-linked immunosorbent assay
Horseradish peroxidase
HRP
O-phenylenediamine
OIA
OPD
Optical immunoassay
PBS
Phosphate buffered saline
Species-specific antibody
SSAb
TA
Tetramethylbenzidine
TMB
Issue Date: 2004
Source: Van, Dong L.,Quyen, L.K.,Gopalakrishnakone, P.,Eng, K.H. (2004). Optical immunoassay for snake venom detection. Biosensors and Bioelectronics 19 (10) : 1285-1294. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2003.11.020
Abstract: A sensitive and specific optical immunoassay (OIA) has been developed for snake venom detection. The assay is based on the principle of detection of physical changes in thickness of molecular thin film resulting from specific binding events on an optical silicon chip (SILAS™-I, ThermoBioStar, Colorado, USA). The reflection of white light through the thin film results in destructive interference of a particular wavelength of the light from gold to purple-blue depending on the thickness of the thin film formed or the amount of venom in the test sample. A prototype test kit for the simultaneous identification of species and semi-quantitative detection of venoms from four medically important snakes of South Vietnam (Trimeresurus albolabris, Calloselasma rhodostoma, Naja kaouthia and Ophiophagus hannah) has been developed. The kit can detect venom analytes in blood, plasma, urine, wound exudates, blister fluid or tissue homogenates. The efficacy of the test kit in snakebite diagnosis has been demonstrated in experimental envenomations and sample analytes taken from snakebite victims in South Vietnam. This rapid snake venom detection kit based on OIA technique is potentially applicable in the clinics as well as in the field. © 2003 Elsevier B.V. All rights reserved.
Source Title: Biosensors and Bioelectronics
URI: http://scholarbank.nus.edu.sg/handle/10635/33862
ISSN: 09565663
DOI: 10.1016/j.bios.2003.11.020
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