Please use this identifier to cite or link to this item: https://doi.org/10.1016/0041-0101(93)90403-6
Title: The black scorpion Heterometrus longimanus: Pharmacological and biochemical investigation of the venom
Authors: Gwee, M.C.E. 
Wong, P.T.-H. 
Gopalakrishnakone, P. 
Cheah, L.S.
Low, K.S.Y. 
Issue Date: 1993
Source: Gwee, M.C.E., Wong, P.T.-H., Gopalakrishnakone, P., Cheah, L.S., Low, K.S.Y. (1993). The black scorpion Heterometrus longimanus: Pharmacological and biochemical investigation of the venom. Toxicon 31 (10) : 1305-1314. ScholarBank@NUS Repository. https://doi.org/10.1016/0041-0101(93)90403-6
Abstract: Documentation on the biological activity (including the lethality) of the venom (BSV) from the black scorpion Heterometrus longimanus is lacking. We have investigated the effects of BSV on adrenergic transmission using the rat isolated anococcygeus muscle (Acm), since the venom from several species of scorpions causes peripheral sympathetic nerve stimulation with enhanced adrenergic responses. The catecholamine content in BSV was also measured by HPLC. The effects of phentolamine (5 μM), guanethidine (5 μM), desipramine (1.5 μM), tetrodotoxin (2 μM) and reserpine pretreatment in vivo (5 mg/kg s.c. x 24 hr and 5 mg/kg i.p. x 3 hr) on contractile responses of the rat Acm to field stimulation, crude BSV (2-10 μl in 6 ml bath), noradrenaline (3 μM), tyramine (10-15 μM), carbachol (2-3 μM) and potassium chloride (50-75 mM) were investigated. BSV mimicked the agonist actions of noradrenaline (NA) by acting directly on postjunctional α-adrenoceptors in the anococcygeus muscle. The LD50 of crude BSV injected i.v. into mice was 0.13 ml per kg mouse. Sequential ultrafiltration of the crude BSV revealed the presence of a substance of low mol. wt which mediates the postjunctional α-agonist action of BSV. HPLC measurements confirmed the presence of noradrenaline (NA; mean concentration of 1.8±0.3 mM) in BSV; the dopamine concentration (mean of 31±4 μM) was 60-fold lower than that of NA, whereas adrenaline was not detected in all the 15 samples investigated. Thus, the presence of NA in BSV can account for the postjunctional α-agonist actions of the venom in the Acm.
Source Title: Toxicon
URI: http://scholarbank.nus.edu.sg/handle/10635/33856
ISSN: 00410101
DOI: 10.1016/0041-0101(93)90403-6
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

18
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

15
checked on Nov 23, 2017

Page view(s)

145
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.