Please use this identifier to cite or link to this item:
|Title:||Pharmacological characterization of mikatoxin, an α-neurotoxin isolated from the venom of the New-Guinean small-eyed snake Micropechis ikaheka|
|Authors:||Nirthanan, S. |
Postsynaptic nicotinic acetylcholine receptor
|Citation:||Nirthanan, S., Gao, R., Gopalakrishnakone, P., Gwee, M.C.E., Khoo, H.E., Cheah, L.S., Kini, R.M. (2002). Pharmacological characterization of mikatoxin, an α-neurotoxin isolated from the venom of the New-Guinean small-eyed snake Micropechis ikaheka. Toxicon 40 (7) : 863-871. ScholarBank@NUS Repository. https://doi.org/10.1016/S0041-0101(01)00268-9|
|Abstract:||Symptoms of envenomation by the New-Guinean small-eyed snake Micropechis ikaheka (Elapidae) include peripheral neurotoxicity and myotoxicity. We have now purified to homogeneity a long-chain neurotoxin, mikatoxin, from M. ikaheka venom by successive gel filtration and reverse-phase chromatography. Electrospray ionization mass spectrometry showed mikatoxin to be a homogenous peptide of MW 7775.6. Mikatoxin was devoid of any phospholipase A2 activity associated with the crude venom and did not exhibit any intrinsic anticholinesterase activity. In the chick biventer cervicis muscle, it produced an irreversible, concentration-dependent block of responses to exogenously applied acetylcholine and carbachol as well as twitches evoked by nerve, but not by direct muscle stimulation. Moreover, mikatoxin, like α-bungarotoxin and erabutoxin-b, did not show significant fade response to train-of-four stimulation of the mouse phrenic nerve-hemi diaphragm muscle. It also failed to block ganglionic transmission in the guinea pig ileum and muscarinic responses in the rat anococcygeus muscle. Our study provides strong evidence for the presence of a neurotoxin (mikatoxin) in M. ikaheka venom that produces neuromuscular blockade in skeletal muscle attributable to selective and irreversible antagonism of postsynaptic nicotinic acetylcholine receptors of the neuromuscular junction and likely contributes to the peripheral neurotoxicity observed in M. ikaheka envenomation. © 2002 Elsevier Science Ltd. All rights reserved.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 23, 2019
WEB OF SCIENCETM
checked on Jan 7, 2019
checked on Jan 13, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.