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|Title:||Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury|
de, Kleijn D.P.V.
El, Oakley R.M.
|Citation:||Lai, R.C., Chen, T.S., Lim, S.K., Arslan, F., Timmers, L., Pasterkamp, G., de, Kleijn D.P.V., Lee, M.M., Choo, A., Sze, N.S.K., Salto-Tellez, M., Lee, C.N., El, Oakley R.M. (2010). Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Research 4 (3) : 214-222. ScholarBank@NUS Repository.|
|Abstract:||Human ESC-derived mesenchymal stem cell (MSC)-conditioned medium (CM) was previously shown to mediate cardioprotection during myocardial ischemia/reperfusion injury through large complexes of 50-100 nm. Here we show that these MSCs secreted 50- to 100-nm particles. These particles could be visualized by electron microscopy and were shown to be phospholipid vesicles consisting of cholesterol, sphingomyelin, and phosphatidylcholine. They contained coimmunoprecipitating exosome-associated proteins, e.g., CD81, CD9, and Alix. These particles were purified as a homogeneous population of particles with a hydrodynamic radius of 55-65 nm by size-exclusion fractionation on a HPLC. Together these observations indicated that these particles are exosomes. These purified exosomes reduced infarct size in a mouse model of myocardial ischemia/reperfusion injury. Therefore, MSC mediated its cardioprotective paracrine effect by secreting exosomes. This novel role of exosomes highlights a new perspective into intercellular mediation of tissue injury and repair, and engenders novel approaches to the development of biologics for tissue repair. © 2009 Elsevier B.V. All rights reserved.|
|Source Title:||Stem Cell Research|
|Appears in Collections:||Staff Publications|
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