Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/33043
Title: TRANSCRIPTOMIC ANALYSIS OF MAMMALIAN SPINAL NEURULATION
Authors: CAO SHOUFENG
Keywords: Laser capture microdissection,Mammalian spinal neurulation,Transcriptomic analysis,High-density oligonucleotide microarray, β-1, 4-galactosyltransfera
Issue Date: 18-Aug-2011
Source: CAO SHOUFENG (2011-08-18). TRANSCRIPTOMIC ANALYSIS OF MAMMALIAN SPINAL NEURULATION. ScholarBank@NUS Repository.
Abstract: Neural tube closure, a process in neurulation, is critical for formation of the central nervous system. Failure of neural tube closure leads to neural tube defects. To identify the molecular regulation of closure, transcriptomic profiling of neurulation was performed using laser capture microdissected neuroepithelium and the surrounding mesenchyme in combination with Affymetrix microarray technology. Various candidate genes involved in regulating median and dorsolateral hinge point formation were identified and groped by signaling pathways and functions based on their Gene Ontology classification. Numerous glycosaminoglycan-related genes, including ß-1, 4-galactosyltransferase which codes for an enzyme involved in initiation of glycosaminoglycan biosynthesis, were found to be differentially expressed in the profiles. The function of this enzyme was investigated using siRNA to silence its expression in NE4C neuroepithelial cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/33043
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Cao SF-PDF.pdf2.61 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

141
checked on Dec 11, 2017

Download(s)

24
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.