Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/32729
Title: Chimeric gene constructs for generation of fluorescent transgenic ornamental fish
Authors: GONG, ZHIYUAN 
HE, JIANGYAN 
JU, BENSHENG 
LAM, TOONG JIN 
XU, YANFEI
YAN, TIE 
Issue Date: 14-Nov-2006
Source: GONG, ZHIYUAN,HE, JIANGYAN,JU, BENSHENG,LAM, TOONG JIN,XU, YANFEI,YAN, TIE (2006-11-14). Chimeric gene constructs for generation of fluorescent transgenic ornamental fish. ScholarBank@NUS Repository.
Abstract: Four zebrafish gene promoters, which are skin specific, muscle specific, skeletal muscle specific and ubiquitously expressed, respectively, were isolated and ligated to the 5' end of the EGFP gene. When the resulting chimeric gene constructs were introduced into zebrafish, the transgenic zebrafish emit green fluorescence under a blue light or ultra-violet light according to the specificity of the promoters used. Thus, new varieties of ornamental fish of different fluorescence patters, e.g., skin fluorescence, muscle fluorescence, skeletal muscle-specific and/or ubiquitous fluorescence, are developed.
URI: http://scholarbank.nus.edu.sg/handle/10635/32729
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