Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/32671
Title: Isolated nucleic acids encoding a secretory signal for expression and secretion of heterologous recombinant proteins
Authors: DING, JEAK LING 
TAN, NGUAN SOON 
HO, BOW 
LAM, TOONG JIN 
Issue Date: 11-May-2004
Source: DING, JEAK LING,TAN, NGUAN SOON,HO, BOW,LAM, TOONG JIN (2004-05-11). Isolated nucleic acids encoding a secretory signal for expression and secretion of heterologous recombinant proteins. ScholarBank@NUS Repository.
Abstract: A universal secretory signal originally derived from a piscine vitellogenin (Vtg) gene is inserted into various expression vectors for driving the secretion of the recombinant protein into the culture medium. This enhances the detection, quantification and downstream scaled-up purification of a recombinant protein of interest. The secretory signal system is very versatile, being conveniently and widely applicable to an array of heterologous host cells such as bacteria, yeast, insect, piscine, and mammalian cell lines (e.g., COS, CHO, NIH/3T3). The said secretory system is also applicable as a reporter vector for secretion of reporter proteins/enzymes, thus, enabling the detection of the reporter proteins (e.g., CAT, GFP) in the culture medium.
URI: http://scholarbank.nus.edu.sg/handle/10635/32671
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