Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/32454
Title: ULTRA-SENSITIVE AND SELECTIVE COLORIMETRIC DNA DETECTION VIA OLIGONUCLEOTIDE-MODIFIED GOLD NANOPARTICLES
Authors: XU WEI
Keywords: DNA, gold nanoparticle, endonuclease, sensor, DNA detection, singnal amplification
Issue Date: 5-Aug-2011
Source: XU WEI (2011-08-05). ULTRA-SENSITIVE AND SELECTIVE COLORIMETRIC DNA DETECTION VIA OLIGONUCLEOTIDE-MODIFIED GOLD NANOPARTICLES. ScholarBank@NUS Repository.
Abstract: The research aim of this thesis is to develop a series of novel and practical DNA/gold nanoparticle (GNP)-based sensors for DNA detection. These sensors combine DNA enzyme reactions and gold nanoparticle amplification, providing high-throughput screening capability and ultra-high sensitivity without the need of costly instrumentation. In the first part, a nicking endonuclease assisted nanoparticle amplification (NEANA) has been developed to detect ultra-long oligonucleotides with 0.5 fmol detection limit and single base mismatch selectivity. In the second part, rolling circle amplification has been employed to couple with NEANA approach for a broadened usage and improved sensitivity. In the third part, restriction endonuclease reaction and ligase chain reaction have been combined for methylated-DNA detection with an exponentially amplified sensitivity in a homogeneous, colorimetric way.
URI: http://scholarbank.nus.edu.sg/handle/10635/32454
Appears in Collections:Ph.D Theses (Open)

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