Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/32385
Title: THE ROLE OF BNIP-2 IN CELL DYNAMICS CONTROL
Authors: LIM GIM KEAT, KENNY
Keywords: BNIP-2, Myosin II, Rho, Mechanobiology, Cell Spreading, Cell Migration
Issue Date: 29-Sep-2011
Source: LIM GIM KEAT, KENNY (2011-09-29). THE ROLE OF BNIP-2 IN CELL DYNAMICS CONTROL. ScholarBank@NUS Repository.
Abstract: Cell attachment to extracellular matrix and the spreading of cell membrane are essential steps leading to cell migration. It requires tight control of cytoskeletal rearrangement and contractile forces mediated by Rho small GTPase and myosin II, an actin based moter protein. We recently identifed BNIP-2 as a novel regulator of Rho leading to cell protrusions and muscle differentiation. However, its function in regulating cell spreading and multicellular/cell-sheet migration remains unclear. Using bioimaging, force-measurement on micropillars, pharmacological inhibition and biochemical assays, we show that the overexpression of BNIP-2 in MDCK cells exerts greater traction force and impairs cell spreading in substrate dependent manner, and it also retards collective cell migration. These findings reveal a novel role of BNIP-2 in regulating distinct Rho signaling underlying cell spreading and cell migration, and could have implications on cell motility during tissue development and cancer metastases.
URI: http://scholarbank.nus.edu.sg/handle/10635/32385
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