Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0006-291X(03)00612-0
Title: IL-10 synergistically enhances GM-CSF-induced CCR1 expression in myelomonocytic cells
Authors: Li, H.
Choo, H.H. 
Chan, J.H.P.
Fred, Wong W.S. 
Cheung, W.
Lai, P.S. 
Keywords: CCR1
GM-CSF
IL-10
LY294002
MIP-1α
p70S6k
PD098059
PKB/Akt
U0126
U937
Issue Date: 2003
Source: Li, H., Choo, H.H., Chan, J.H.P., Fred, Wong W.S., Cheung, W., Lai, P.S. (2003). IL-10 synergistically enhances GM-CSF-induced CCR1 expression in myelomonocytic cells. Biochemical and Biophysical Research Communications 304 (2) : 417-424. ScholarBank@NUS Repository. https://doi.org/10.1016/S0006-291X(03)00612-0
Abstract: CC chemokine receptor 1 (CCR1) has been implicated in inflammation. The present study examined the signaling mechanisms that mediate GM-CSF/IL-10-induced synergistic CCR1 protein expression in monocytic U937 cells. GM-CSF alone markedly increased both the mRNA and protein expression of CCR1. IL-10 augmented GM-CSF-induced CCR1 protein expression with no effect on mRNA expression. PD098059 and U0126 (two MEK inhibitors), and LY294002 (a PI3K inhibitor) inhibited GM-CSF/IL-10-induced CCR1 gene and protein expression. PD098059, U0126, and LY294002 also attenuated chemotaxis of GM-CSF/IL-10-primed U937 cells in response to MIP-1α. Immunoblotting studies show that GM-CSF alone induced ERK2 phosphorylation; whereas, IL-10 alone induced p70S6k phosphorylation in U937 cells. Neither cytokine when used alone induced PKB/Akt phosphorylation. Combined GM-CSF/IL-10 treatment of U937 cells induced phosphorylation of ERK2, p70S6k, and PKB/Akt. PD098059 and U0126 completely abrogated ERK2 phosphorylation; whereas, LY294002 completely blocked PKB/Akt and p70S6k phosphorylation. Our findings indicate that IL-10 may potentiate GM-CSF-induced CCR1 protein expression in U937 cells via activation of PKB/Akt and p70S6k. © 2003 Elsevier Science (USA). All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/32274
ISSN: 0006291X
DOI: 10.1016/S0006-291X(03)00612-0
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