Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0167-0115(00)00162-2
Title: Effects of des-aspartate-angiotensin I on angiotensin II-induced incorporation of phenylalanine and thymidine in cultured rat cardiomyocytes and aortic smooth muscle cells
Authors: Min, L.
Sim, M.K. 
Xu, X.G.
Keywords: Cardiomyocytes
Losartan
Peptides
Vascular smooth muscle cells
Issue Date: 2000
Source: Min, L., Sim, M.K., Xu, X.G. (2000). Effects of des-aspartate-angiotensin I on angiotensin II-induced incorporation of phenylalanine and thymidine in cultured rat cardiomyocytes and aortic smooth muscle cells. Regulatory Peptides 95 (1-3) : 93-97. ScholarBank@NUS Repository. https://doi.org/10.1016/S0167-0115(00)00162-2
Abstract: Des-aspartate-angiotensin I, a pharmacologically active nine-amino acid angiotensin peptide, and losartan, an AT1 angiotensin receptor antagonist, but not angiotensin-(1-7), another active angiotensin peptide, completely attenuated the angiotensin II-induced incorporation of [3H]phenylalanine in cultured rat cardiomyocytes. The attenuation by des-aspartate-angiotensin I but not that of losartan was inhibited by indomethacin. The data support an earlier suggestion that the nonapeptide attenuates cardiac hypertrophy in rats via an indomethacin-sensitive angiotensin AT1 receptor subtype. In rat aortic smooth muscle cells, both des-aspartate-angiotensin I and angiotensin-(1-7) had no effect on the angiotensin II-induced [3H]phenylalanine incorporation. However, the two peptides significantly attenuated the angiotensin II-induced [3H]thymidine incorporation in the smooth muscle cells. The attenuation by angiotensin-(1-7) but not by des-aspartate-angiotensin I was inhibited by (D-Ala7)-angiotensin-(1-7), a specific angiotensin-(1-7) antagonist. Des-aspartate-angiotensin I also attenuated FCS-stimulated [3H]thymidine incorporation. This attenuation was inhibited by the peptide angiotensin receptor antagonist, (Sar1,Ile8)-angiotensin II, but not by losartan. These data indicate that des-aspartate-angiotensin I and angiotensin-(1-7) do not participate in the process of protein synthesis in vascular smooth muscle cells and that the nonapeptide and heptapeptide act on different non-AT1 receptors to mediate their anti-hyperplasic action. Although the exact mechanisms of action remain to be elucidated, the findings indicate that des-aspartate-angiotensin I acts as an agonist on angiotensin AT1 and non-AT1 receptor subtypes and induces responses that oppose the actions of angiotensin II. (C) 2000 Elseveir Science B.V.
Source Title: Regulatory Peptides
URI: http://scholarbank.nus.edu.sg/handle/10635/32152
ISSN: 01670115
DOI: 10.1016/S0167-0115(00)00162-2
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