Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0021-9673(96)00901-6
Title: High-performance liquid chromatographic determination of dehydroabietic and abietic acids in traditional Chinese medications
Authors: Lee, B.L.
Koh, D. 
Ong, H.Y.
Ong, C.N. 
Keywords: Abietic acid
Colophony
Dehydroabietic acid
Organic acids
Pharmaceutical analysis
Issue Date: 1997
Citation: Lee, B.L., Koh, D., Ong, H.Y., Ong, C.N. (1997). High-performance liquid chromatographic determination of dehydroabietic and abietic acids in traditional Chinese medications. Journal of Chromatography A 763 (1-2) : 221-226. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(96)00901-6
Abstract: In Asia, there is still a high usage of traditional Chinese medicament by the general population. Some patients with contact dermatitis to these medicaments have been found to be sensitive to colophony on patch testing. Dehydroabietic acid (DHAA) and abietic acid (AA) are the main components of colophony and believed to be the agents responsible for skin sensitization. This paper describes a reliable high-performance liquid-chromatographic method for determining these two resin acids in ointment samples. The samples were either pretreated with diethyl ether or treated with acetonitrile directly by ultrasonication for 30 min. One volume of this sample was added to an equal volume of water and purified by solid-phase extraction. The mobile phase used was methanol-water-phosphoric acid (87:13:0.02, v/v) and the flow-rate was 1 ml/min. DHAA and AA were detected at 4.3 and 6.3 min with ultraviolet detection at wavelength 200 and 239 nm, respectively. However, fluorimetric detection with an excitation wavelength of 225 nm and emission wavelength of 285 nm, provided more selective determination of DHAA. The detection limits for DHAA and AA were 1 ng. Analytical recovery generally exceeded 90%. We analyzed nine types of commonly used topical Chinese medicaments and two types of Western medical ointments in Singapore. The results showed that most of these medicaments contain colophony below 5 ppm (μg g-1). Only one Chinese medicament contained >70 ppm of both allergens and one of the Western medical ointments contained 0.2% of DHAA and 2.2% of AA.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/31897
ISSN: 00219673
DOI: 10.1016/S0021-9673(96)00901-6
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

15
checked on Jul 17, 2018

WEB OF SCIENCETM
Citations

15
checked on Jun 27, 2018

Page view(s)

141
checked on May 12, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.