Please use this identifier to cite or link to this item:
|Title:||Monomeric C18chromatographic method for the liquid chromatographic determination of lipophilic antioxidants in plants|
|Source:||Lee, B.L.,Su, J.,Ong, C.N. (2004). Monomeric C18chromatographic method for the liquid chromatographic determination of lipophilic antioxidants in plants. Journal of Chromatography A 1048 (2) : 263-267. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2004.07.057|
|Abstract:||Reversed-phase liquid chromatography was used to determine lipophilic antioxidants in plants using two monomeric C18 columns operated at 30°C and 4°C, with a column-switching technique and acetonitrile- methanol gradient elution. The chromatograms were extracted at different wavelengths using a UV diode array detector (DAD). A wide range of plant antioxidants, including nine carotenoids (neoxanthin, violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, lycopene, canthaxanthin, α-carotene and β-carotene) together with all-trans-retinol, capsaicin, dihydrocapsaicin, chlorophyll a, and chlorophyll b can be separated within 50 min. Fluorometric detection was applied to quantify trace amounts of six vitamin E analogues (α-, δ- and γ-tocopherols and tocotrienols). The detection limits were 0.2-0.4 μg/g for various xanthophylls and 0.04-0.10 μg/g for vitamin E analogues. © 2004 Elsevier B.V. All rights reserved.|
|Source Title:||Journal of Chromatography A|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 12, 2017
checked on Dec 15, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.