Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.chroma.2004.07.057
Title: Monomeric C18chromatographic method for the liquid chromatographic determination of lipophilic antioxidants in plants
Authors: Lee, B.L.
Su, J.
Ong, C.N. 
Keywords: Antioxidants
Column switching
Vitamins
Issue Date: 2004
Source: Lee, B.L.,Su, J.,Ong, C.N. (2004). Monomeric C18chromatographic method for the liquid chromatographic determination of lipophilic antioxidants in plants. Journal of Chromatography A 1048 (2) : 263-267. ScholarBank@NUS Repository. https://doi.org/10.1016/j.chroma.2004.07.057
Abstract: Reversed-phase liquid chromatography was used to determine lipophilic antioxidants in plants using two monomeric C18 columns operated at 30°C and 4°C, with a column-switching technique and acetonitrile- methanol gradient elution. The chromatograms were extracted at different wavelengths using a UV diode array detector (DAD). A wide range of plant antioxidants, including nine carotenoids (neoxanthin, violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, lycopene, canthaxanthin, α-carotene and β-carotene) together with all-trans-retinol, capsaicin, dihydrocapsaicin, chlorophyll a, and chlorophyll b can be separated within 50 min. Fluorometric detection was applied to quantify trace amounts of six vitamin E analogues (α-, δ- and γ-tocopherols and tocotrienols). The detection limits were 0.2-0.4 μg/g for various xanthophylls and 0.04-0.10 μg/g for vitamin E analogues. © 2004 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/31830
ISSN: 00219673
DOI: 10.1016/j.chroma.2004.07.057
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