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|Title:||Automated high-performance liquid chromatographic method with precolumn reduction for the determination of ubiquinol and ubiquinone in human plasma|
|Source:||Wang, Q., Lee, B.L., Ong, C.N. (1999). Automated high-performance liquid chromatographic method with precolumn reduction for the determination of ubiquinol and ubiquinone in human plasma. Journal of Chromatography B: Biomedical Sciences and Applications 726 (1-2) : 297-302. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-4347(99)00067-5|
|Abstract:||We developed a gradient HPLC method with automated precolumn reduction for direct electrochemical detection of ubiquinol-10 (CoQ10H2) and total coenzyme Q10 (TQ10) in human plasma. The concentration of ubiquinone-10 (CoQ10) was calculated by subtraction of CoQ10H2 from TQ10. Preparation of reducing agent and precolumn reduction was performed by a programmable auto-injector. The two mobile phases used were: A, 100% of methanol containing 50 mM sodium perchlorate and 10 mM perchloric acid; and B, a mixture of ethanol and tert.-butanol (80:20, v/v). Sample preparation was simply a deproteinisation process with 10-fold ethanol. A good linear relationship was obtained for CoQ10H2 concentration from 0.1 to 3 μmol/l. The detection limit was 2.5 nmol/l with an injection volume of 20 μl. The analytical recovery and reproduciblity were generally >90%. To validate the method, 18 freshly collected plasma samples of normal healthy subjects were analysed. The mean ratio of CoQ10H2/CoQ10 was 93:7. The proposed method is sensitive, reliable and can be used for clinical investigation. Copyright (C) 1999 Elsevier Science B.V.|
|Source Title:||Journal of Chromatography B: Biomedical Sciences and Applications|
|Appears in Collections:||Staff Publications|
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