Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-4347(92)80445-V
Title: High-performance liquid chromatographic method for routine determination of vitamins A and E and β-carotene in plasma
Authors: Lee, B.L.
Chua, S.C. 
Ong, H.Y.
Ong, C.N. 
Issue Date: 1992
Source: Lee, B.L., Chua, S.C., Ong, H.Y., Ong, C.N. (1992). High-performance liquid chromatographic method for routine determination of vitamins A and E and β-carotene in plasma. Journal of Chromatography - Biomedical Applications 581 (1) : 41-47. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-4347(92)80445-V
Abstract: A simple and reliable reversed-phase high-performance liquid chromatographic (HPLC) method for the routine determination of vitamins A and E and β-carotene in plasma (or serum) with wavelength-programmed ultraviolet-visible absorbance detection is described. A 200-μl aliquot of serum or plasma sample, after deproteinization with ethanol, and containing tocopherol acetate as internal standard, was extracted with butanol-ethyl acetate. Sodium sulphate was added for dehydration. Analytes of extracted samples were found to be stable for at least four days. A 10-μl aliquot of this organic extract was used for HPLC analysis. The mobile phase was methanol-butanol-water (89.5:5:5.5, v/v) and the flow-rate was set at 1.5 ml/min. The analytes of interest were well separated from other plasma constituents within 22 min at 45°C. The lowest detection limits of vitamins A and E and β-carotene were 0.02, 0.5 and 0.1 μg/ml, respectively. The recovery and reproducibility of the present method were around 90%. The method is sensitive, specific and can be used for epidemiological studies and for routine determination of vitamin deficiency. Several important factors that may affect the analysis are also discussed in this paper.
Source Title: Journal of Chromatography - Biomedical Applications
URI: http://scholarbank.nus.edu.sg/handle/10635/31715
ISSN: 03784347
DOI: 10.1016/0378-4347(92)80445-V
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

147
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

130
checked on Dec 13, 2017

Page view(s)

147
checked on Dec 19, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.