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https://scholarbank.nus.edu.sg/handle/10635/31596
DC Field | Value | |
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dc.title | THE ZEBRAFISH PLASMA MEMBRANE CA2+ PUMP ATP2B1A ACTS IN THE DEVELOPMENT OF MECHANORECEPTORS AND EARLY BONE CALCIFICATION | |
dc.contributor.author | GO TECK WAH WILLIAM | |
dc.date.accessioned | 2012-03-31T18:00:49Z | |
dc.date.available | 2012-03-31T18:00:49Z | |
dc.date.issued | 2011-08-24 | |
dc.identifier.citation | GO TECK WAH WILLIAM (2011-08-24). THE ZEBRAFISH PLASMA MEMBRANE CA2+ PUMP ATP2B1A ACTS IN THE DEVELOPMENT OF MECHANORECEPTORS AND EARLY BONE CALCIFICATION. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/31596 | |
dc.description.abstract | Tol2 enhancer-trap transgenic line SqET4 is widely used for studies of development and disease of mechanosensory hair cells. We showed that it represents Tg:atp2b1a-EGFP. This provided a possibility to study a function of Atp2b1a/PMCA1. Combining the morpholino mediated-knockdown and small molecule inhibition, I showed that deficiency of Atp2b1a results in auditory and vestibular defects due to defects of Ca2+ export by mechanosensory hair cells. Acting downstream of the transcriptional regulator Atoh1a, Atp2b1a plays a crucial role in division of terminally determined mechanosensory hair cells progenitors. Serendipitously, another domain of GFP/Atp2b1a expression was identified in the ultimobranchial bodies (UB) of SqET4. In mammals UB gives rise to the calcitonin-producing cells of the thyroid gland. Since in zebrafish UB remains a separate organ, SqET4 provided a useful tool to study the anatomy and function of developing UB. In particular, a connection between UB and pharyngeal dentition and function of Atp2b1a during bones calcification was demonstrated. | |
dc.language.iso | en | |
dc.subject | zebrafish,enhancer trap,mechanosensory,ultimobranchial body,calcitonin,plasma membrane calcium pump, | |
dc.type | Thesis | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.supervisor | HONG WAN JIN | |
dc.contributor.supervisor | KORZH, VLADIMIR | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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GoTWW.pdf | 13.77 MB | Adobe PDF | OPEN | None | View/Download |
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