Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/31596
Title: THE ZEBRAFISH PLASMA MEMBRANE CA2+ PUMP ATP2B1A ACTS IN THE DEVELOPMENT OF MECHANORECEPTORS AND EARLY BONE CALCIFICATION
Authors: GO TECK WAH WILLIAM
Keywords: zebrafish,enhancer trap,mechanosensory,ultimobranchial body,calcitonin,plasma membrane calcium pump,
Issue Date: 24-Aug-2011
Source: GO TECK WAH WILLIAM (2011-08-24). THE ZEBRAFISH PLASMA MEMBRANE CA2+ PUMP ATP2B1A ACTS IN THE DEVELOPMENT OF MECHANORECEPTORS AND EARLY BONE CALCIFICATION. ScholarBank@NUS Repository.
Abstract: Tol2 enhancer-trap transgenic line SqET4 is widely used for studies of development and disease of mechanosensory hair cells. We showed that it represents Tg:atp2b1a-EGFP. This provided a possibility to study a function of Atp2b1a/PMCA1. Combining the morpholino mediated-knockdown and small molecule inhibition, I showed that deficiency of Atp2b1a results in auditory and vestibular defects due to defects of Ca2+ export by mechanosensory hair cells. Acting downstream of the transcriptional regulator Atoh1a, Atp2b1a plays a crucial role in division of terminally determined mechanosensory hair cells progenitors. Serendipitously, another domain of GFP/Atp2b1a expression was identified in the ultimobranchial bodies (UB) of SqET4. In mammals UB gives rise to the calcitonin-producing cells of the thyroid gland. Since in zebrafish UB remains a separate organ, SqET4 provided a useful tool to study the anatomy and function of developing UB. In particular, a connection between UB and pharyngeal dentition and function of Atp2b1a during bones calcification was demonstrated.
URI: http://scholarbank.nus.edu.sg/handle/10635/31596
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