Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/31585
Title: Isolation of broadly cross-reactive human antibodies against dengue virus using a human non-immunized fab phage display library
Authors: CHAN HOI YI
Keywords: Dengue, human antibody, phage display, prM protein, envelope protein
Issue Date: 4-Jan-2011
Source: CHAN HOI YI (2011-01-04). Isolation of broadly cross-reactive human antibodies against dengue virus using a human non-immunized fab phage display library. ScholarBank@NUS Repository.
Abstract: Recent studies have indicated that the human antibody response to DENV targets epitopes that differ from mouse; hence, human antibodies are essential for relevant study of DENV. The flexibility and diversity of phage display technology allow for selection of antibodies with a desired specificity/cross-specificity. The goal of this project was to isolate complex-reactive anti-DENV antibodies by screening all 4 DENV serotypes sequentially using a non-immunized human phage display library. One unique clone, D29, was converted into the human IgG1 format and was reactive against the prM of all 4 DENV serotypes. It was able to potently enhance immature DENV2 (imDENV2) infection in Fc?R-bearing cells across a broad concentration range. Co-localization of furin with D29-imDENV2 immune-complex supported the critical involvement of furin in the anti-prM mediated enhancement. This study has shown that phage display library is a useful tool that can complement the existing techniques in human antibody isolation.
URI: http://scholarbank.nus.edu.sg/handle/10635/31585
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01 ChanHYA Title page.pdf5.3 kBAdobe PDF

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02 ChanHYA Acknowledgements merged.pdf190.1 kBAdobe PDF

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03 ChanHYA Introduction n method n result n discussion n blibliography.pdf3.19 MBAdobe PDF

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