Please use this identifier to cite or link to this item:
|Title:||A new era in pyrogen testing|
|Authors:||Ding, J.L. |
|Citation:||Ding, J.L., Ho, B. (2001). A new era in pyrogen testing. Trends in Biotechnology 19 (8) : 277-281. ScholarBank@NUS Repository.|
|Abstract:||Pyrogens are substances (usually of biological origin) that cause fever after injection. The best-studied pyrogen is lipopolysaccharide (LPS, also known as endotoxin), found in the membrane of Gram-negative bacteria. During Gram-negative sepsis, endotoxin stimulates host macrophages to release inflammatory cytokines and excessive inflammation causes multiple organ failure and death. Endotoxins are thus ubiquitous pathogenic molecules that are a bane to the pharmaceutical industry and medical community. Limulus amoebocyte lysate (LAL) has been widely used for ∼25 years for the detection of endotoxin in quality control of injectable drugs and medical devices. However, variations in sensitivity and specificity of LAL to endotoxin, and the limited supply of limulus (horseshoe crabs) has called for an alternative pyrogen test. Recombinant Factor C (rFC), the endotoxin-inducible coagulation enzyme in LAL, forms the basis of a novel micro-enzymatic assay for high-throughput screens of endotoxin and opens a new era in endotoxin testing. Endotoxin activates the rFC zymogen, which catalytically hydrolyses synthetic substrates to form measurable products, thus quantifying the endotoxin.|
|Source Title:||Trends in Biotechnology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 28, 2018
WEB OF SCIENCETM
checked on Apr 23, 2018
checked on Nov 17, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.