Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.febslet.2004.08.087
Title: Identification of a novel shrimp protein phosphatase and its association with latencey-related ORF427 of white spot syndrome virus
Authors: Lu, L.
Kwang, J. 
Keywords: Latency
Lysosome
ORF427
Protein phosphatase
WSSV
Issue Date: 2004
Source: Lu, L., Kwang, J. (2004). Identification of a novel shrimp protein phosphatase and its association with latencey-related ORF427 of white spot syndrome virus. FEBS Letters 577 (1-2) : 141-146. ScholarBank@NUS Repository. https://doi.org/10.1016/j.febslet.2004.08.087
Abstract: To characterize the role of latency-associated ORF427 of white spot syndrome virus (WSSV), a shrimp cDNA library was constructed to screen interacting proteins of ORF427. Employing the yeast two-hybrid system, a novel shrimp protein phosphatase (named PPs), sharing 93% homology with human protein phosphatase 1, has been identified able to bind ORF427 in yeast. Through co-immunoprecipitation assays, the interaction between PPs and ORF427 was further confirmed both in vitro and in vivo. Interestingly, the novel shrimp protein phosphatase consists of only 199 aa and contains almost all the functional catalytic domains of human protein phosphatase, while it lacks the corresponding C-terminal non-catalytic sequence. Transcription and translation products of the identified cDNA can be detected in both normal and WSSV-infected shrimps; and PPs was found to localize mainly in the lysosome of shrimp cells. To characterize its function, the PPs cDNA was highly expressed in bacteria and the purified protein showed phosphatase activity when tested against pNPP in a standard phosphatase assay. Our results suggest that the identified protein phosphatase, PPs, may represent a novel member of protein phosphatase family and might be involved in the regulation of WSSV's life cycle through interaction with latency-related ORF427 of WSSV. © 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Source Title: FEBS Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/31241
ISSN: 00145793
DOI: 10.1016/j.febslet.2004.08.087
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

27
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

27
checked on Nov 23, 2017

Page view(s)

144
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.