Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.vaccine.2004.09.015
Title: Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production
Authors: Ho, P.S.
Lee, Y.K. 
Kwang, J. 
Keywords: Coronavirus
Oral vaccination
Spike glycoprotein
Issue Date: 2005
Source: Ho, P.S., Lee, Y.K., Kwang, J. (2005). Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 23 (11) : 1335-1342. ScholarBank@NUS Repository. https://doi.org/10.1016/j.vaccine.2004.09.015
Abstract: Lactobacillus casei strain Shirota was selected as a bacterial carrier for the development of live mucosal vaccines against coronavirus. A 75 kDa fragment of transmissible gastroenteritis coronavirus (TGEV) spike glycoprotein S was used as the model coronavirus antigen. The S glycoprotein was cloned into a Lactobacillus/E. coli shuttle vector (pLP500) where expression and secretion of the glycoprotein S from the recombinant lactobacilli was detected via immunoblotting. Oral immunization of BALB/c mice with recombinant LcS that constitutively expresses the 75 kDa fragment of the glycoprotein S, induced both local mucosal and systemic immune responses against TGEV. Maximum titers of IgG (8.38 ± 0.19 ng/ml of serum) and IgA (64.82 ± 2.9 ng/ml of intestinal water) were attained 32 days post oral inturbation. The induced antibodies demonstrated neutralizing effects on TGEV infection. © 2004 Elsevier Ltd. All rights reserved.
Source Title: Vaccine
URI: http://scholarbank.nus.edu.sg/handle/10635/31234
ISSN: 0264410X
DOI: 10.1016/j.vaccine.2004.09.015
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