Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.mimet.2004.03.008
Title: Isolation and characterization of the mitochondrion-like organelle from Blastocystis hominis
Authors: Nasirudeen, A.M.A. 
Tan, K.S.W. 
Keywords: Blastocystis
Mitochondrial transmembrane potential
Mitochondrion
Protozoa
Rhodamine 123
Issue Date: 2004
Source: Nasirudeen, A.M.A., Tan, K.S.W. (2004). Isolation and characterization of the mitochondrion-like organelle from Blastocystis hominis. Journal of Microbiological Methods 58 (1) : 101-109. ScholarBank@NUS Repository. https://doi.org/10.1016/j.mimet.2004.03.008
Abstract: Blastocystis hominis in an unusual protozoan parasite of the human intestinal tract. Previous studies have described the presence of mitochondrial-like structures despite the anaerobic nature of the organism. In this study, we describe a simple and rapid technique to isolate and characterize mitochondrion-like organelles (MLO) from B. hominis. The parasite was disrupted using glass beads and the MLO were collected and purified using a sucrose gradient. Negative staining and transmission electron microscopy of the isolated organelles showed mitochondrial-like structures. B. hominis cells were stained with rhodamine 123 and MitoLight™ to show the presence of transmembrane potential of the MLO. DAPI staining of the cells suggested the presence of DNA in the MLO. Though brief reports have been made in literature, this study is the first to describe a technique for the isolation of the MLO from this organism. Using this technique of isolation, major metabolic functions of the organelle, their associated macromolecules and intra-mitochondrial location can be extensively studied. The role of MLO in this anaerobic protozoan can be widely investigated using this protocol. © 2004 Elsevier B.V. All rights reserved.
Source Title: Journal of Microbiological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/31173
ISSN: 01677012
DOI: 10.1016/j.mimet.2004.03.008
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

32
checked on Dec 5, 2017

WEB OF SCIENCETM
Citations

30
checked on Nov 16, 2017

Page view(s)

107
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.