Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/31172
Title: Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2)
Authors: Tan, A.L.
Loke, P. 
Sim, T.-S. 
Keywords: D-ala-D-ala dipeptidase
Streptomyces coelicolor
Vancomycin resistance
vanX
Issue Date: 2002
Citation: Tan, A.L., Loke, P., Sim, T.-S. (2002). Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2). Research in Microbiology 153 (1) : 27-32. ScholarBank@NUS Repository.
Abstract: The vanX gene which encodes a D-alanyl-D-alanine dipeptidase is critical for vancomycin resistance in enterococci. A putative vanX gene from Streptomyces coelicolor A3(2), which is not known for vancomycin production, was identified by homology-based analysis and cloned by polymerase chain reaction. The S. coelicolor vanX gene was heterologously expressed in Escherichia coli BL21(DE3) and enzymatic assays of soluble protein fractions of VanX revealed a 93-fold increase in dipeptidase activity as compared to the nonrecombinant control, thus confirming its functionality. Interestingly, S. coelicolor was also found to be of intermediate resistance to vancomycin although it does not produce vancomycin, thus suggesting the role of VanX in defence or immunity. As such, the prevalence of vanX genes in the environment may be more common than previously thought. © 2002 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.
Source Title: Research in Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/31172
ISSN: 09232508
Appears in Collections:Staff Publications

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