Please use this identifier to cite or link to this item:
|Title:||In vitro conversion of penicillin G and ampicillin by recombinant Streptomyces clavuligerus NRRL 3585 deacetoxycephalosporin C synthase|
Deacetoxycephalosproin C synthase
|Source:||Sim, J., Sim, T.-S. (2001). In vitro conversion of penicillin G and ampicillin by recombinant Streptomyces clavuligerus NRRL 3585 deacetoxycephalosporin C synthase. Enzyme and Microbial Technology 29 (4-5) : 240-245. ScholarBank@NUS Repository. https://doi.org/10.1016/S0141-0229(01)00377-5|
|Abstract:||The in vitro conversion of penicillin N analogues to cephalosporins was first demonstrated in resting cells and cell-free extracts of Streptomyces clavuligerus NP1. This study has optimized the conversion rate of a highly expressed soluble source of recombinant S. clavuligerus NRRL 3585 deacetoxycepalosporin C synthase (scDAOCS) through detailed characterization of its cofactor requirements. Inclusion of dithiothreitol (DTT) and ascorbate at various concentrations from 0.2-16 mM and 0.2-10 mM was found to be inhibitory to scDAOCS activity under specified reaction conditions examined. Simultaneous omission of these cofactors in a new modified reaction condition, comprising of 50 mM Tris HCl (pH7.4), 0.8 mM ATP, 1.28 mM α-ketoglutarate and 1.8 mM FeSO4, enhanced the conversion rate of two penicillin N analogues, penicillin G and ampicillin, through 20- and 35-fold respectively. © 2001 Elsevier Science Inc. All rights reserved.|
|Source Title:||Enzyme and Microbial Technology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 7, 2017
WEB OF SCIENCETM
checked on Nov 22, 2017
checked on Dec 10, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.