Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/30945
DC Field | Value | |
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dc.title | COMPARISON OF CYTOSINE DEAMINASE/5-FLUOROCYTOSINE VERSUS HERPES SIMPLEX VIRUS THYMIDINE KINASE/GANCICLOVIR ENZYME/PRODRUG SYSTEMS IN GLIOBLASTOMA GENE THERAPY. | |
dc.contributor.author | YE KAI | |
dc.date.accessioned | 2012-03-15T18:00:13Z | |
dc.date.available | 2012-03-15T18:00:13Z | |
dc.date.issued | 2011-08-19 | |
dc.identifier.citation | YE KAI (2011-08-19). COMPARISON OF CYTOSINE DEAMINASE/5-FLUOROCYTOSINE VERSUS HERPES SIMPLEX VIRUS THYMIDINE KINASE/GANCICLOVIR ENZYME/PRODRUG SYSTEMS IN GLIOBLASTOMA GENE THERAPY.. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/30945 | |
dc.description.abstract | Cytosine deaminase (CD)/5-fluorocytosine (5-FC) and herpes simplex virus thymidine kinase (HSVtk)/ganciclovir (GCV) systems are the most well-studied and extensively used suicide gene/prodrug systems in cancer gene therapy. In this study, we evaluated and compared the inhibitory effects of HSVtk/GCV and CD/5-FC on glioma development. In vitro results indicate that when delivered by suicide gene expression in the U87 glioma cell line and in neural stem cells (NSCs), the CD/5-FC system was able to induce a bystander killing effect stronger than that of the HSVtk/GCV system, thus being more effective in eliminating glioma cells. Intratumoral injection of NSCs expressing the CD gene into BALB/c nude mice harboring U87 glioma xenografts induced significant tumor regression, and tumor growth was inhibited when 5-FC was administered. Bacterial CD/5-FC and yeast CD/5-FC displayed similar anti-glioma effects in vitro and in vivo. These results suggested that the antiglioma effect of the CD/5-FC system is superior to the HSVtk/GCV system, with the former being more suitable for glioma gene therapy when used with NSCs as a delivery vehicle. | |
dc.language.iso | en | |
dc.subject | Glioblastoma Gene Therapy CD\5FC HSVtk\GCV Neural Stem Cell | |
dc.type | Thesis | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.supervisor | WANG SHU | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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YeK.pdf | 1.03 MB | Adobe PDF | OPEN | None | View/Download |
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