Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/30684
Title: Developing High-Throughput Chemical Approaches For Proteomic Profiling Of Aspartic Proteases And Protein Kinases
Authors: SHI HAIBIN
Keywords: high-throughput, proteomic profiling, affinity based probe, aspartic protease, protein kinase
Issue Date: 24-Jul-2011
Source: SHI HAIBIN (2011-07-24). Developing High-Throughput Chemical Approaches For Proteomic Profiling Of Aspartic Proteases And Protein Kinases. ScholarBank@NUS Repository.
Abstract: To reach the goal of large scale studies of a significant number of enzymes present in any particular organism, development of high-throughput amenable chemistry and enzyme-screening tools are becoming very urgent. This thesis examines and addresses these challenges by combination of various synthetic approaches and introducing high throughput screening platforms. Chapter 2 describes a solution-cum-solid phase strategy for rapid assembly of diol-containing small molecules as potential HIV-1 inhibitors. Chapter 3 presents a high throughput synthetic strategy for rapid assembly of 475-member aspartic protease inhibitors library using ?click? reaction. With this platform, new inhibitors against malaria were discovered. Chapter 4 and 5 present a small molecule microarray-facilated platform for proteome profiling of aspartic proteases in cellular lysates. Chapter 6 and 7 describes the cell permeable, photo-affinity drug-like probes for proteome profiling of cellular on/off-targets of drug dasatinib and staurosporine.
URI: http://scholarbank.nus.edu.sg/handle/10635/30684
Appears in Collections:Ph.D Theses (Open)

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