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https://doi.org/10.1016/S0959-8049(03)00231-4
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dc.title | Intrinsic variability in the detection of micrometastases in lymph nodes for re-staging of colorectal cancer: Effect of individual markers and tissue samples | |
dc.contributor.author | Salto-Tellez, M. | |
dc.contributor.author | Kong, S.L. | |
dc.contributor.author | Koay, E.S.C. | |
dc.contributor.author | Leong, A.P.K. | |
dc.date.accessioned | 2012-02-01T05:24:35Z | |
dc.date.available | 2012-02-01T05:24:35Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Salto-Tellez, M., Kong, S.L., Koay, E.S.C., Leong, A.P.K. (2003). Intrinsic variability in the detection of micrometastases in lymph nodes for re-staging of colorectal cancer: Effect of individual markers and tissue samples. European Journal of Cancer 39 (9) : 1234-1241. ScholarBank@NUS Repository. https://doi.org/10.1016/S0959-8049(03)00231-4 | |
dc.identifier.issn | 09598049 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/30414 | |
dc.description.abstract | In this study, we investigated whether (a) carcinoembryonic antigen (CEA), cytokeratin-20 (CK-20) and guanylyl cyclase C (GCC) are clinically useful markers for the molecular detection of submicroscopic metastases in colorectal cancer (CRC) and (b) whether overexpression of CEA, CK-20 and GCC can be reliably detected in formalin-fixed, paraffin-embedded tissues as well as frozen lymph nodes. We studied 175 frozen lymph nodes and 158 formalin-fixed, paraffin-embedded lymph nodes from 28 cases of CRC. CEA or CK-20 or GCC-specific polymerase chain reaction (PCR) was carried out on mRNA transcripts extracted from the nodal tissues. Ten out of 11 Dukes' B CRC cases had detectable CEA and CK-20 while 6 out of 11 Dukes' B CRC cases had detectable GCC. In general, the difference of re-staged cases when comparing frozen and paraffin-embedded samples was marked; the only statistically significant correlation between frozen and paraffin tissue was for the CEA marker. Our results indicated a high incidence (>50%) of detecting micrometastases in histologically-negative lymph nodes at the molecular level. © 2003 Elsevier Science Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0959-8049(03)00231-4 | |
dc.source | Scopus | |
dc.subject | CEA | |
dc.subject | CK-20 | |
dc.subject | Colorectal cancer | |
dc.subject | GCC | |
dc.subject | Lymph nodes | |
dc.subject | Micrometastases | |
dc.type | Article | |
dc.contributor.department | PATHOLOGY | |
dc.description.doi | 10.1016/S0959-8049(03)00231-4 | |
dc.description.sourcetitle | European Journal of Cancer | |
dc.description.volume | 39 | |
dc.description.issue | 9 | |
dc.description.page | 1234-1241 | |
dc.description.coden | EJCAE | |
dc.identifier.isiut | 000183427600018 | |
Appears in Collections: | Staff Publications |
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