Please use this identifier to cite or link to this item:
|Title:||Intrinsic variability in the detection of micrometastases in lymph nodes for re-staging of colorectal cancer: Effect of individual markers and tissue samples|
|Authors:||Salto-Tellez, M. |
|Source:||Salto-Tellez, M.,Kong, S.L.,Koay, E.S.C.,Leong, A.P.K. (2003). Intrinsic variability in the detection of micrometastases in lymph nodes for re-staging of colorectal cancer: Effect of individual markers and tissue samples. European Journal of Cancer 39 (9) : 1234-1241. ScholarBank@NUS Repository. https://doi.org/10.1016/S0959-8049(03)00231-4|
|Abstract:||In this study, we investigated whether (a) carcinoembryonic antigen (CEA), cytokeratin-20 (CK-20) and guanylyl cyclase C (GCC) are clinically useful markers for the molecular detection of submicroscopic metastases in colorectal cancer (CRC) and (b) whether overexpression of CEA, CK-20 and GCC can be reliably detected in formalin-fixed, paraffin-embedded tissues as well as frozen lymph nodes. We studied 175 frozen lymph nodes and 158 formalin-fixed, paraffin-embedded lymph nodes from 28 cases of CRC. CEA or CK-20 or GCC-specific polymerase chain reaction (PCR) was carried out on mRNA transcripts extracted from the nodal tissues. Ten out of 11 Dukes' B CRC cases had detectable CEA and CK-20 while 6 out of 11 Dukes' B CRC cases had detectable GCC. In general, the difference of re-staged cases when comparing frozen and paraffin-embedded samples was marked; the only statistically significant correlation between frozen and paraffin tissue was for the CEA marker. Our results indicated a high incidence (>50%) of detecting micrometastases in histologically-negative lymph nodes at the molecular level. © 2003 Elsevier Science Ltd. All rights reserved.|
|Source Title:||European Journal of Cancer|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 13, 2017
WEB OF SCIENCETM
checked on Nov 4, 2017
checked on Dec 9, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.