Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/30282
Title: Study of Pharmacokinetics of Prenylflavonoids and dynamics of estrogen action in sera following ingestion of Epimedium using validated, ultra-sensitive cell-based bioassays
Authors: WONG SHIH PENG
Keywords: Epimedium, prenylflavonid, phytoestrogen, bioassay, pharmacokinetics, estrogen receptor
Issue Date: 8-Mar-2011
Source: WONG SHIH PENG (2011-03-08). Study of Pharmacokinetics of Prenylflavonoids and dynamics of estrogen action in sera following ingestion of Epimedium using validated, ultra-sensitive cell-based bioassays. ScholarBank@NUS Repository.
Abstract: Epimedium is consumed to improve menopausal health. Its constituents are metabolized to known or unknown compounds which may be estrogenic but data on their combined effects are sparse. Cell lines that permanently express ERa and ER?, and MCF-7 breast cancer cell proliferation assay were developed and validated, and used to correlate pharmacokinetics of prenylflavonoids with the dynamics of their combined estrogenicities of sera obtained from human volunteers and rats after consumption of a traditionally prepared Epimedium decoction and a prenylflavonoid-enriched formulation, respectively. Ex-vivo sera samples did not exert significant estrogenicity due to extensive in-vivo conjugation of Epimedium prenylflavonoids. Gene expression profiling was also utilized to fish for genes that are differentially regulated by Epimedium extract and its compounds. The standardized Epimedium extract, icariside I, icariside II, icaritin and desmethylicaritin were found to upregulate the CYP1A1 gene. In contrast, cells treated with estadiol and genistein did not show a similar upregulation.
URI: http://scholarbank.nus.edu.sg/handle/10635/30282
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2. SUMMARY.pdf198.44 kBAdobe PDF

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3. INTRODUCTION.pdf1.37 MBAdobe PDF

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4. METHODS AND MATERIALS.pdf364.61 kBAdobe PDF

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5. RESULTS.pdf2.06 MBAdobe PDF

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6. DISCUSSION.pdf291.26 kBAdobe PDF

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7. CONCLUSION.pdf196.61 kBAdobe PDF

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8. BIBLIOGRAPHY.pdf259.17 kBAdobe PDF

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