Please use this identifier to cite or link to this item:
|Title:||Generating mESC-derived insulin-producing cell lines through an intermediate lineage-restricted progenitor line|
|Authors:||Li, G. |
|Citation:||Li, G., Luo, R., Zhang, J., Lian, Q., Xie, F., Lim, S.K., Salto-Tellez, M., Yeo, K.S., Tan, E.K.W., Caille, D., Meda, P., Kon, O.L. (2009). Generating mESC-derived insulin-producing cell lines through an intermediate lineage-restricted progenitor line. Stem Cell Research 2 (1) : 41-55. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2008.07.006|
|Abstract:||Generating surrogate insulin-producing cells from embryonic stem cells (ESCs) through in vitro replication of successive steps during pancreatic development has been challenging . Here we describe a novel reproducible protocol to establish homogeneous and scalable insulin-producing cell lines from mouse (m) ESCs via differentiation of the previously described lineage-restricted clonal mESC-derived E-RoSH cells. Unlike their parental mESCs, E-RoSH cells expressed high levels of mesodermal and endodermal genes. Nutrient depletion in the presence of nicotinamide inhibited proliferation of E-RoSH cells and induced differentiation into heterogeneous cultures comprising vascular-like structures that produced detectable levels of insulin and C-peptide in an equimolar ratio. Limiting dilution of these cultures resulted in the isolation of eight independent insulin-producing cell lines in five experiments. All these lines were cloned and shown to be amenable to repeated cycles of freeze and thaw and to replicate for months with a doubling time of 3-4 days. Under such conditions, the cultured cells exhibited genomic, structural, biochemical, and pharmacological properties of pancreatic β cells, including storage of an equimolar ratio of insulin and C-peptide in granules and release of the contents of these organelles through a glucose-sensitive machinery. After transplantation, these cells reversed hyperglycemia in streptozotocin-treated SCID mice and did not form teratomas. © 2008.|
|Source Title:||Stem Cell Research|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 15, 2018
WEB OF SCIENCETM
checked on Oct 8, 2018
checked on Jul 28, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.