Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/29943
Title: CONJUGATED POLYMER BASED FLUORESCENT NANOPARTICLES FOR BIOIMAGING
Authors: LI KAI
Keywords: conjugated polymer, nanoparticles, fluorescence, cancer cell, bioimaging, target
Issue Date: 30-May-2011
Source: LI KAI (2011-05-30). CONJUGATED POLYMER BASED FLUORESCENT NANOPARTICLES FOR BIOIMAGING. ScholarBank@NUS Repository.
Abstract: Fluorescent probes have emerged as a class of promising imaging reagents in cancer cell detection as well as broad biological imaging applications. During past decades, organic dye and quantum dots (QDs) have been highly relied on for fluorescence imaging studies. Recently, a novel class of fluorescent nanoparticles, conjugated polymer based fluorescent nanoparticles (CP NPs), have been reported to show high brightness, good photostability and low cytotoxicity in cellular imaging studies, which make them promising alternatives to conventional fluorescent probes. However, surface functionalized CP NPs with good targeting ability to specific cancer cells and intracellular biomolecules remain lack for CP NP based bioimaging assays. In this thesis, various CP NPs with desired fluorescence properties, surface functionalities, and low cytotoxicity have been developed for targeted cancer cell imaging and intracellular target detection. Firstly, a general and feasible strategy to fabricate biocompatible and surface-amendable conjugated polymer based fluorescent nanoparticles has been developed and their application in targeted cancer cell imaging has been demonstrated. Following that, polyhedral oligomeric silsesquioxanes (POSS) based conjugated polymer has been used to improve the quantum yield of CP NPs. Further conjugation of these nanoparticles with antibody allowed for detection of targeted cells with good selectivity. Subsequent efforts were made to simultaneously discriminate different cancer cells in mixed cell suspension under single laser wavelength excitation. At last, intracellular biotarget detection in living cells were carried out using water-soluble hyperbranched conjugated polymer nanospheres with small size, good living cell membrane permeability and targeting ligand functionalization.
URI: http://scholarbank.nus.edu.sg/handle/10635/29943
Appears in Collections:Ph.D Theses (Open)

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