Please use this identifier to cite or link to this item:
https://doi.org/10.1016/S0142-9612(03)00404-6
DC Field | Value | |
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dc.title | Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance | |
dc.contributor.author | Lu, H.-F. | |
dc.contributor.author | Lim, W.S. | |
dc.contributor.author | Wang, J. | |
dc.contributor.author | Tang, Z.-Q. | |
dc.contributor.author | Zhang, P.-C. | |
dc.contributor.author | Leong, K.W. | |
dc.contributor.author | Mao, H.-Q. | |
dc.contributor.author | Chia, S.M. | |
dc.contributor.author | Yu, H. | |
dc.date.accessioned | 2011-12-07T08:17:03Z | |
dc.date.available | 2011-12-07T08:17:03Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Lu, H.-F., Lim, W.S., Wang, J., Tang, Z.-Q., Zhang, P.-C., Leong, K.W., Mao, H.-Q., Chia, S.M., Yu, H. (2003). Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance. Biomaterials 24 (27) : 4893-4903. ScholarBank@NUS Repository. https://doi.org/10.1016/S0142-9612(03)00404-6 | |
dc.identifier.issn | 01429612 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/29738 | |
dc.description.abstract | One of the major challenges in BLAD design is to develop functional substrates suitable for hepatocyte attachment and functional maintenance. In the present study, we designed a poly(vinylidene difluoride) (PVDF) surface coated with galactose-tethered Pluronic polymer. The galactose-derived Pluronic F68 (F68-Gal) was adsorbed on PVDF membrane through hydrophobic-hydrophobic interaction between PVDF and the polypropylene oxide segment in Pluronic. The galactose density on the modified PVDF surface increased with the concentration of the F68-Gal solution, reaching 15.4nmol galactosyl groups per cm2 when a 1mg/ml of F68-Gal solution was used. The adsorbed F68-Gal remained relatively stable in culture medium. Rat hepatocytes attachment efficiency on F68-Gal modified PVDF membrane was similar to that on collagen-coated surface. The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These attached hepatocytes in spheroids exhibited higher cell functions such as albumin synthesis and P450 1A1 detoxification function compared to unmodified PVDF membrane and collagen-coated surface. These results suggest the potential of this galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. © 2003 Elsevier Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0142-9612(03)00404-6 | |
dc.source | Scopus | |
dc.subject | Bioartificial liver assist device | |
dc.subject | Galactose immobilization | |
dc.subject | Hepatocyte culture | |
dc.subject | PVDF membrane | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.department | MATERIALS SCIENCE | |
dc.description.doi | 10.1016/S0142-9612(03)00404-6 | |
dc.description.sourcetitle | Biomaterials | |
dc.description.volume | 24 | |
dc.description.issue | 27 | |
dc.description.page | 4893-4903 | |
dc.description.coden | BIMAD | |
dc.identifier.isiut | 000186267600001 | |
Appears in Collections: | Staff Publications |
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