Please use this identifier to cite or link to this item:
|Title:||Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance|
|Keywords:||Bioartificial liver assist device|
|Citation:||Lu, H.-F., Lim, W.S., Wang, J., Tang, Z.-Q., Zhang, P.-C., Leong, K.W., Mao, H.-Q., Chia, S.M., Yu, H. (2003). Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance. Biomaterials 24 (27) : 4893-4903. ScholarBank@NUS Repository. https://doi.org/10.1016/S0142-9612(03)00404-6|
|Abstract:||One of the major challenges in BLAD design is to develop functional substrates suitable for hepatocyte attachment and functional maintenance. In the present study, we designed a poly(vinylidene difluoride) (PVDF) surface coated with galactose-tethered Pluronic polymer. The galactose-derived Pluronic F68 (F68-Gal) was adsorbed on PVDF membrane through hydrophobic-hydrophobic interaction between PVDF and the polypropylene oxide segment in Pluronic. The galactose density on the modified PVDF surface increased with the concentration of the F68-Gal solution, reaching 15.4nmol galactosyl groups per cm2 when a 1mg/ml of F68-Gal solution was used. The adsorbed F68-Gal remained relatively stable in culture medium. Rat hepatocytes attachment efficiency on F68-Gal modified PVDF membrane was similar to that on collagen-coated surface. The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These attached hepatocytes in spheroids exhibited higher cell functions such as albumin synthesis and P450 1A1 detoxification function compared to unmodified PVDF membrane and collagen-coated surface. These results suggest the potential of this galactose-immobilized PVDF membrane as a suitable substrate for hepatocyte culture. © 2003 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 14, 2018
WEB OF SCIENCETM
checked on Nov 7, 2018
checked on Nov 10, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.