Please use this identifier to cite or link to this item:
|Title:||Regulation of anterior pituitary galanin gene expression by thyroid hormone|
|Authors:||Hooi, S.C. |
|Citation:||Hooi, S.C., Koenig, J.I., Abraczinskas, D.R., Kaplan, L.M. (1997). Regulation of anterior pituitary galanin gene expression by thyroid hormone. Molecular Brain Research 51 (1-2) : 15-22. ScholarBank@NUS Repository.|
|Abstract:||Thyroid hormone is required for basal and estrogen-induced expression of anterior pituitary galanin. Steady-state anterior pituitary galanin mRNA levels decreased 6-fold in hypothyroid rats after 3 weeks of treatment. Similarly, hypothyroidism resulted in a 2.6-fold decrease in estrogen induction of galanin gene expression. The effect of thyroid hormone on anterior pituitary galanin gene expression appears to be exerted, at least in part, at the pituitary itself. Transient expression assays in GH3 cells suggest the involvement of transcriptional mechanisms in the regulation of galanin gene expression by thyroid hormone. A region between -41 and -132 bp upstream of the transcriptional start site confers thyroid hormone responsiveness to the galanin gene. Gel-mobility shift assays show specific binding of 'SPI-like' proteins in GH3 nuclear extracts to this region of the galanin gene. This binding was greatly enhanced by thyroid hormone.|
|Source Title:||Molecular Brain Research|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 18, 2018
WEB OF SCIENCETM
checked on Dec 24, 2018
checked on Dec 23, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.