Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/29551
Title: Transcriptome Analysis of Human Embryonal Carcinoma Cell Lines
Authors: CHAK LI LING
Keywords: transcriptome, human embryonal carcinoma, microarray, reverseSAGE
Issue Date: 30-Mar-2011
Source: CHAK LI LING (2011-03-30). Transcriptome Analysis of Human Embryonal Carcinoma Cell Lines. ScholarBank@NUS Repository.
Abstract: Embryonal carcinoma cell lines (ECCs) isolated from the undifferentiated cell component of teratocarcinomas were important for early studies on pluripotent stem cells and paved the way for the eventual establishment of human embryonic stem cell lines (hESCs). Human ECCs are the malignant counterpart to hESCs, and hECC cell lines with different levels of differentiation ability, from nullipotent to pluripotent, have been established. Due to biosafety concerns in using hESCs for cell therapy, there was a keen interest to determine and interpret the gene expression profile in association to biological activity observed in hECCs and hESCs. Thus, a transcriptome approach was used to study the coding profile of hECCs and hESCs by DNA microarray and the noncoding RNA (ncRNA) profile by reverseSAGE. hECCs and hESCs were shown to share significant expression of the ES signature and cell surface molecules involved in cancer metastasis. In comparison to hESCs, hECCs also had more genes associated with cell cycle and transcription processes, with several of these linked to cancer development. Large amounts of ncRNA, which includes antisense transcription, was confirmed to be present in hECCs through reverseSAGE. Knockdown study of long ncRNA HEST147 in hECCs showed possible involvement in cell proliferation regulation. In conclusion, expression of the ES signature may potentially contribute towards tumourigenicity and warrants the continual study of hECCs to understand the formation of tumours from pluripotent stem cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/29551
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