Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/29518
Title: MULTIVALENT MHC BLOCKERS TARGETING HLA-DQ2 ANTIGEN-PRESENTATION
Authors: RAYMOND TING-GEE YAN
Keywords: Multivalent,HLA-DQ2,gliadin,celiac,dendrimer,peptide synthesis
Issue Date: 23-Feb-2010
Source: RAYMOND TING-GEE YAN (2010-02-23). MULTIVALENT MHC BLOCKERS TARGETING HLA-DQ2 ANTIGEN-PRESENTATION. ScholarBank@NUS Repository.
Abstract: The aim of this project is to design multivalent MHC blockers as competitive inhibitors of HLA-DQ2 antigen presentation. The implications of such inhibitors serve as a therapeutic agent towards the onset of autoimmunity in celiac disease. Our initial approach is to create a small multivalent library consisting of dimers, trimers, tetramers, and octamers. These multivalent constructs are covalently linked to an a-I gliadin analog (wheat gluten peptide) through a thiol-reactive core molecule (i.e. multivalent linkers). These multivalent constructs will be used to test the multivalent hypothesis, which assumes that a molecule with more copies of binding ligand will favor its binding affinity. To verify such hypothesis, biochemical binding assay with HLA-DQ2 will be performed, followed by T cell proliferation assay to measure blocking efficiencies. This study begins to address the important issue regarding structural optimization (i.e. linker length and valency) in rational design for achieving competitive MHC blocking through a multivalent strategy.
URI: http://scholarbank.nus.edu.sg/handle/10635/29518
Appears in Collections:Master's Theses (Open)

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