Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jdermsci.2004.11.009
Title: Viability and adipogenic potential of human adipose tissue processed cell population obtained from pump-assisted and syringe-assisted liposuction
Authors: Leong, D.T.W.
Chew, F.T. 
Hutmacher, D.W. 
Lim, T.-C. 
Keywords: Adipogenic
Autologous lipoinjection
Fat grafting
Pump-assisted liposuction
Issue Date: 2005
Source: Leong, D.T.W., Chew, F.T., Hutmacher, D.W., Lim, T.-C. (2005). Viability and adipogenic potential of human adipose tissue processed cell population obtained from pump-assisted and syringe-assisted liposuction. Journal of Dermatological Science 37 (3) : 169-176. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jdermsci.2004.11.009
Abstract: Background: Correcting soft tissue defects by autologous fat grafting has been a routine procedure in plastic surgery. Liposuction material for autologous fat grafting is normally obtained using a hand-held syringe. The pump-assisted liposuction technique is not used because of the belief that cells in the harvested fat tissue are necrotic from the physical forces created by the vacuum pump liposuction machine. Objective: The metabolism and adipogenic potential of cultured mesenchymal precursor cells processed from pump and syringe lipoaspirates were studied. Methods: Metabolic estimates were determined with alamarBlue™ assay. These cells were also induced along the adipogenic lineage with adipogenic induction factors. The extent of adipogenic differentiation was measured using a digital photo counting software. Results: The cells obtained by pump-assisted liposuction are viable, have comparable metabolic activity and adipogenic potential which are comparable to cells using the syringe for aspiration. Conclusion: The implications of this study are that pump-assisted liposuction might be a possible alternative to aspirating adipose tissue for reimplantation during reconstruction procedures. © 2004 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.
Source Title: Journal of Dermatological Science
URI: http://scholarbank.nus.edu.sg/handle/10635/29448
ISSN: 09231811
DOI: 10.1016/j.jdermsci.2004.11.009
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