Please use this identifier to cite or link to this item:
|Title:||Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+mobilization|
|Citation:||Wei, H., Song, J., Fang, L., Chatterjee, S., Li, G. (2004). Identification of a novel transcript of human PECAM-1 and its role in the transendothelial migration of monocytes and Ca2+mobilization. Biochemical and Biophysical Research Communications 320 (4) : 1228-1235. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2004.05.226|
|Abstract:||Platelet-endothelial cell adhesion molecule-1 (PECAM-1) is an integral component of endothelial cells and has been implicated in the transendothelial migration (TEM) of circulating leukocytes mediated by its 1st and 2nd extracellular immunoglobulin (Ig)-like domains and regulation of intracellular Ca2+ homeostasis with its 6th domain. Up-to-date, little is known about the role of the 5th extracellular (Ig)-like domain. We have discovered a novel human PECAM-1 transcript missing the entire 7th exon, which encodes the 5th extracellular (Ig)-like domain of PECAM-1. A synthetic peptide with sequence homology to the 5th domain of PECAM-1 (JHS-7 peptide) and a corresponding polyclonal antibody (JHS-7 Ab) were prepared and their potential role in transendothelial migration and Ca2+ influx was measured. The JHS-7 peptide and the antibody exerted a dose dependent decrease (50-80%) in the transendothelial migration of freshly isolated human monocytes and a promonocytic cell line (U-937) in resting HUVECs and HUVECs activated with tumor necrosis factor-α. This was accompanied by an increase in Ca2+ influx and decrease in refilling of the intracellular Ca2+ stores in HUVECs. In summary, we have identified a novel PECAM-1 transcript (Δexon 7) and shown that the 5th (Ig)-like domain of PECAM-1 plays a role in monocyte TEM and Ca2+ homeostasis. © 2004 Elsevier Inc. All rights reserved.|
|Source Title:||Biochemical and Biophysical Research Communications|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 19, 2018
WEB OF SCIENCETM
checked on Apr 10, 2018
checked on May 12, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.