Please use this identifier to cite or link to this item:
|Title:||Identification of CD8+T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus|
|Authors:||Yu, H. |
|Source:||Yu, H., Chan, S., Srinivasan, N., Ren, E. (2005). Identification of CD8+T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus. Human Immunology 66 (5) : 483-493. ScholarBank@NUS Repository. https://doi.org/10.1016/j.humimm.2005.01.017|
|Abstract:||Nasopharyngeal carcinoma (NPC) is a human epithelial tumor with a high incidence in Southern Chinese population, with contributions from Epstein-Barr virus (EBV), human leukocyte antigen (HLA), and environmental factors to its etiology. It has been shown previously that the recognition of immediate-early transactivator Rta of EBV by CD8+ T cells may have a significant impact on controlling EBV and, indirectly, NPC. The current study used two computer-aided prediction methods and competition-based HLA-peptide binding assays to screen for HLA B2704/B4601/B5801 restricted T-cell epitopes derived from Rta. HLA tetrameric complexes containing these potential T-cell epitopes were synthesized. Rta-specific CD8+ T-cell responses in healthy virus carriers were then defined by these tetramers and IFN-γ ELISPOT assays. We clearly demonstrated that healthy virus carriers have detectable Rta-specific CD8+ T cells restricted by B2704 in the circulation. However, there were no B4601/B5801 tetramer-reactive T cells specific for Rta in the peripheral blood of matched/mismatched donors. On the other hand, B4601 tetramers containing the computer-predicted B4601 binder EBNA3A (318-326) showed detectable tetramer-reactive T cells in the circulation of healthy virus carriers. topes also elicited IFN-γ responses as detected by ELISPOT. © American Society for Histocompatibility and Immunogenetics, 2005. Published by Elsevier Inc.|
|Source Title:||Human Immunology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 6, 2017
WEB OF SCIENCETM
checked on Nov 21, 2017
checked on Dec 10, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.