Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2008.06.052
Title: Rational design, solution conformation and identification of functional residues of the soluble and structured Nogo-54, which mimics Nogo-66 in inhibiting the CNS neurite outgrowth
Authors: Li, M. 
Liao, X.
Qin, H.
Song, J. 
Li, Y.
Xiao, Z.-c.
Liu, J. 
Keywords: Antagonist design
Circular dichroism
CNS injury
Neurite outgrowth
NMR spectroscopy
Nogo
Nogo-66 receptor
Solution conformation
Issue Date: 2008
Source: Li, M., Liao, X., Qin, H., Song, J., Li, Y., Xiao, Z.-c., Liu, J. (2008). Rational design, solution conformation and identification of functional residues of the soluble and structured Nogo-54, which mimics Nogo-66 in inhibiting the CNS neurite outgrowth. Biochemical and Biophysical Research Communications 373 (4) : 498-503. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.06.052
Abstract: The interaction between Nogo-66 and its receptor NgR represents a promising target for designing drugs to treat CNS axonal injury which often leads to permanent disability. Unfortunately, the isolated Nogo-66 is highly insoluble while its truncated form Nogo-40 is soluble but unstructured, thus retarding further characterization and application. Here, we rationally design another soluble form Nogo-54. CD and NMR characterization reveals that Nogo-54 is structured, and importantly, is able to mimic Nogo-66 in inhibiting neurite outgrowth. Strikingly, mutating its C-terminal four residues (Lys50, Glu51, Arg53, and Arg54) leads to a mutant Nogo-54m which has no dramatic structural change but whose inhibitory activity is completely abolished. This strongly suggests that the four charged residues contribute significantly to the inhibitory action of Nogo-66. Furthermore, our study also provides a soluble and structured mimic as well as a possible antagonist for Nogo-66 which may hold promising potential for various medical applications. © 2008 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/28904
ISSN: 0006291X
10902104
DOI: 10.1016/j.bbrc.2008.06.052
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

5
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

5
checked on Nov 29, 2017

Page view(s)

114
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.