Please use this identifier to cite or link to this item:
|Title:||Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide|
|Authors:||Whiteman, M. |
Reactive oxygen species
|Citation:||Whiteman, M., Chu, S.H., Siau, J.L., Li, L., Bhatia, M., Moore, P.K., Kostetski, I. (2006). Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide. Biochemical and Biophysical Research Communications 343 (1) : 303-310. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2006.02.154|
|Abstract:||The gaseous mediators hydrogen sulphide (H2S) and nitric oxide (.NO) are synthesised in the body from l-cysteine and l-arginine, respectively. In the cardiovascular system, .NO is an important regulator of vascular tone and its over- or under-production has been linked to a variety of diseases. The physiological significance of H2S is not yet clear but, like .NO, it exhibits vasodilator activity and may play a part in septic and haemorrhagic shock, hypertension, regulation of cardiac contractility, and in inflammation. To date, there have been no reports of a chemical interaction between H2S and .NO. Here we show that incubation of the H2S donor, sodium hydrosulphide, with a range of .NO donors and .NO gas in vitro leads to the formation of a nitrosothiol molecule as determined by a combination of techniques; electron paramagnetic resonance, amperometry, and measurement of nitrite. We further show that this nitrosothiol did not induce cGMP accumulation in cultured RAW264.7 cells unless .NO was released with Cu 2+. Finally, using liver homogenates from LPS treated rats we present evidence for the endogenous formation of this nitrosothiol. These findings provide the first evidence for the formation of a novel nitrosothiol generated by reaction between H2S and .NO. We propose that generation of this nitrosothiol in the body may regulate the physiological effects of both .NO and H2S. © 2006 Elsevier Inc. All rights reserved.|
|Source Title:||Biochemical and Biophysical Research Communications|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 8, 2018
WEB OF SCIENCETM
checked on Jul 31, 2018
checked on Jul 13, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.