Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.taap.2005.04.010
Title: Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells
Authors: Rose, P. 
Whiteman, M.
Huang, Q.
Ong, C.N. 
Keywords: Chemoprevention
Cruciferous vegetables
Invasion
Isothiocyanate
Matrix metalloproteinase
Issue Date: 2005
Source: Rose, P., Whiteman, M., Huang, Q., Ong, C.N. (2005). Broccoli and watercress suppress matrix metalloproteinase-9 activity and invasiveness of human MDA-MB-231 breast cancer cells. Toxicology and Applied Pharmacology 209 (2) : 105-113. ScholarBank@NUS Repository. https://doi.org/10.1016/j.taap.2005.04.010
Abstract: A high dietary intake of cruciferous vegetables has been associated with a reduction in numerous human pathologies particularly cancer. In the current study, we examined the inhibitory effects of broccoli (Brassica oleracea var. italica) and watercress (Rorripa nasturtium aquaticum) extracts on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cancer cell invasion and matrix metalloproteinase-9 activity using human MDA-MB-231 breast cancer cells. Aberrant overexpression of matrix metalloproteinases, including metalloproteinase-9, is associated with increased invasive potential in cancer cell lines. Our results demonstrate that extracts of broccoli and Rorripa suppressed TPA-induced MMP-9 activity and invasiveness in a concentration dependant manner as determined by zymographic analysis. Furthermore, fractionation of individual extracts followed by liquid chromatography mass spectroscopy analysis (LC-MS) revealed that the inhibitory effects of each vegetable were associated with the presence of 4-methysulfinylbutyl (sulforaphane) and 7-methylsulphinylheptyl isothiocyanates. Taken together, our data indicate that isothiocyanates derived form broccoli and Rorripa inhibit metalloproteinase 9 activities and also suppress the invasive potential of human MDA-MB-231 breast cancer cells in vitro. The inhibitory effects observed in the current study may contribute to the suppression of carcinogenesis by diets high in cruciferous vegetables. © 2005 Elsevier Inc. All rights reserved.
Source Title: Toxicology and Applied Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/28805
ISSN: 0041008X
10960333
DOI: 10.1016/j.taap.2005.04.010
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