Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2009.02.136
Title: A novel approach to the identification and quantitative elemental analysis of amyloid deposits-Insights into the pathology of Alzheimer's disease
Authors: Rajendran, R.
Minqin, R.
Watt, F. 
Ynsa, M.D.
Casadesus, G.
Smith, M.A.
Perry, G.
Halliwell, B. 
Keywords: Alzheimer's disease
Copper
Iron
PIXE
RBS
STIM
Zinc
Issue Date: 2009
Source: Rajendran, R., Minqin, R., Watt, F., Ynsa, M.D., Casadesus, G., Smith, M.A., Perry, G., Halliwell, B. (2009). A novel approach to the identification and quantitative elemental analysis of amyloid deposits-Insights into the pathology of Alzheimer's disease. Biochemical and Biophysical Research Communications 382 (1) : 91-95. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2009.02.136
Abstract: There is considerable interest in the role of metals such as iron, copper, and zinc in amyloid plaque formation in Alzheimer's disease. However to convincingly establish their presence in plaques in vivo, a sensitive technique is required that is both quantitatively accurate and avoids isolation of plaques or staining/fixing brain tissue, since these processes introduce contaminants and redistribute elements within the tissue. Combining the three ion beam techniques of scanning transmission ion microscopy, Rutherford back scattering spectrometry and particle induced X-ray emission in conjunction with a high energy (MeV) proton microprobe we have imaged plaques in freeze-dried unstained brain sections from CRND-8 mice, and simultaneously quantified iron, copper, and zinc. Our results show increased metal concentrations within the amyloid plaques compared with the surrounding tissue: iron (85 ppm compared with 42 ppm), copper (16 ppm compared to 6 ppm), and zinc (87 ppm compared to 34 ppm). © 2009 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/28789
ISSN: 0006291X
10902104
DOI: 10.1016/j.bbrc.2009.02.136
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